Mechanism of action
Why identify your drugs' mechanism(s) of action?
Early detection of a compound’s mechanism of action speeds its optimization at the pertinent target(s). Better understanding of your drugs' mechanism of action helps refine your drugs' indication and define drugs' contingency plan.
Which technologies to use
Oxygen consumption ATP production Glycolysis level TCA cycle turning Cell viability ROS mitochondrial production Cytoplasm ROS accumulation MnSOD Cu/ZnSOD activities Catalase activity Total GSH activity Lipid peroxidation level (TBARS) Protein carbonylation Cell viability Changes in mitochondrial mtDNA content, which have been reported in a broad range of diseases and is a pertinent target in diabetes, obesity, cancer, and HIV complications. Mitochondrial autophagy
Mitophagy is the selective degradation of mitochondria by autophagy. ICDD provides image analyses to quantify this phenomenon.
Quantitative analysis of mitochondrial proteins or of proteins modulating mitochondrial functions.