Anti-oxidation & mitochondria
Excessive production of reactive oxygen species (ROS) is one of the causes of aging.
Mitochondria are the primary site of ROS production. Mitochondria also control the first lines of anti-oxidant defenses and regulate cellular ROS scavengers activity. ROS are dynamically controlled in the cell as they contribute to inflammation signaling and to protein and DNA damages leading to cell injury. As a result, ROS participate in cell reactivity/adaptation, but can also contribute to cell damages.
Measure the effect of your ingredients at the basal level or after oxidative stress induction.
We have several chemical or physical stressors available to mimic different increases in oxidative stress. We can mimic the effects of:
Age Pollution Sun exposure with UV, IR and/or heat effect
Our assays have been developed in several human primary cell models (fibroblasts, keratinocytes, etc.) and can be adapted to meet your specific needs.
Which technologies to use
The Redox status package demonstrates anti-oxidant action following physical stress such as IR-, UV-, or heat-exposure.
This package includes the measurement of (any or all):
Cellular and mitochondrial radical oxygen species (ROS) production MnSOD and cSOD activities Total Gluthation activity Cellular catalase activity Lipid peroxidation level (TBARs) Protein carbonylation